[10] Assay of inorganic phosphate, total phosphate and phosphatases
BN Ames - Methods in enzymology, 1966 - Elsevier
Publisher Summary This chapter discusses the assay of inorganic phosphate, total
phosphate, and phosphatases. The phosphomolybdate complex is reduced by ascorbic
acid. The method is about seven times as sensitive as the Fiske–SubbaRow procedure and
involves less pipetting. One can easily determine 0.01 micromole of phosphate.
Pyrophosphate breaks down about 5% in the method and compounds such as glucose 1-
phosphate also break down somewhat, so that the method is not very satisfactory for …
phosphate, and phosphatases. The phosphomolybdate complex is reduced by ascorbic
acid. The method is about seven times as sensitive as the Fiske–SubbaRow procedure and
involves less pipetting. One can easily determine 0.01 micromole of phosphate.
Pyrophosphate breaks down about 5% in the method and compounds such as glucose 1-
phosphate also break down somewhat, so that the method is not very satisfactory for …
