Metabolic fate of nitric oxide
K Yoshida, K Kasama, M Kitabatake, M Okuda… - International archives of …, 1980 - Springer
K Yoshida, K Kasama, M Kitabatake, M Okuda, M Imai
International archives of occupational and environmental health, 1980•SpringerThe metabolic pathway of inhaled NO in rats was investigated with 15 NO. After 15 NO
exposure, the content of the 15 N (atom% excess) in blood, red cells, serum, each organ
tissue (perfused with saline solution) and urine were estimated. The contents of 15 N in the
samples of blood, serum, red cells, and urine were relatively high and those of lung, trachea,
liver, kidney, and muscle were low. The rat blood samples 1 h after 15 NO combined blood
injection were analyzed. Greater parts of the 15 N in the serum were in a fraction of the …
exposure, the content of the 15 N (atom% excess) in blood, red cells, serum, each organ
tissue (perfused with saline solution) and urine were estimated. The contents of 15 N in the
samples of blood, serum, red cells, and urine were relatively high and those of lung, trachea,
liver, kidney, and muscle were low. The rat blood samples 1 h after 15 NO combined blood
injection were analyzed. Greater parts of the 15 N in the serum were in a fraction of the …
Summary
The metabolic pathway of inhaled NO in rats was investigated with 15NO. After 15NO exposure, the content of the 15N (atom % excess) in blood, red cells, serum, each organ tissue (perfused with saline solution) and urine were estimated. The contents of 15N in the samples of blood, serum, red cells, and urine were relatively high and those of lung, trachea, liver, kidney, and muscle were low.
The rat blood samples 1 h after 15NO combined blood injection were analyzed. Greater parts of the 15N in the serum were in a fraction of the ultrafiltrate and the amounts of NO 3 − in the serum were remarkably elevated compared with those of the control rats.
At 24 h and 48 h after 15NO combined blood injection, the urine samples were collected. Most of the 15N in the urine was found within 24 h.
Red cells from the 15NO exposed rats were washed (mixed and incubated) with the saline solution or the serum from untreated rats. The 15N in the cells was easily extracted into the solution or serum, but the 15N in the serum from the exposed animals was scarcely transferred to the red cells.
Throughout the experiments, it was supposed that inhaled NO primarily reacted with hemoglobin and was changed to NO 2 − and NO 3 − . Then the metabolites were transferred to serum, part of these reacted with tissues and the others were excreted in urine in the form of NO 3 − .
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