The carboxy terminus of the. beta. amyloid protein is critical for the seeding of amyloid formation: Implications for the pathogenesis of Alzheimer's disease
JT Jarrett, EP Berger, PT Lansbury Jr - Biochemistry, 1993 - ACS Publications
JT Jarrett, EP Berger, PT Lansbury Jr
Biochemistry, 1993•ACS PublicationsMATERIALS AND METHODS Synthesis and Purification of Peptides. The peptides 026-39,
026-40, 026-42, and 026-43 were synthesized on the PAM resin using standard
(BOC/benzyl) syntheticprotocols. Peptide-resin was deprotected with TFMSA/dimethyl sul-
fide/TFA (1: 3: 5) at0 C for 2 h, and the resin was washed with dichloromethane and dried in
vacuo. The peptide was removed from the resin with TFMS A/thioanisole/m-cresol/TFA (1: 1:
1: 10) at 0 C for 1 h, and the supernatant was precipitated andwashed with diethyl ether. The …
026-40, 026-42, and 026-43 were synthesized on the PAM resin using standard
(BOC/benzyl) syntheticprotocols. Peptide-resin was deprotected with TFMSA/dimethyl sul-
fide/TFA (1: 3: 5) at0 C for 2 h, and the resin was washed with dichloromethane and dried in
vacuo. The peptide was removed from the resin with TFMS A/thioanisole/m-cresol/TFA (1: 1:
1: 10) at 0 C for 1 h, and the supernatant was precipitated andwashed with diethyl ether. The …
MATERIALS AND METHODS
Synthesis and Purification of Peptides. The peptides 026-39, 026-40, 026-42, and 026-43 were synthesized on the PAM resin using standard (BOC/benzyl) syntheticprotocols.
Peptide-resin was deprotected with TFMSA/dimethyl sul-fide/TFA (1: 3: 5) at0 C for 2 h, and the resin was washed with dichloromethane and dried in vacuo. The peptide was removed from the resin with TFMS A/thioanisole/m-cresol/TFA (1: 1: 1: 10) at 0 C for 1 h, and the supernatant was precipitated andwashed with diethyl ether. The peptides 026-39 and 026-40 were dissolved in DMSO and purified by RPHPLC (purity> 90% by isocratic elution). The peptides 026-42 and 026-43, due to their insolubility in water, were purified by gel permeation HPLC in hexafluoro-2-propanol (HFIP)(Hendrix et al., 1992). Peptides were analyzed by PDMS and produced the expected parent ion. 2 3 The purity
ACS Publications