Amino acid sequence based PCR primers for amplification of rearranged human heavy and light chain immunoglobulin variable region genes
M Welschof, P Terness, F Kolbinger, M Zewe… - Journal of …, 1995 - Elsevier
Previously described primers for PCR amplification of variable immunoglobulin (Ig) genes
were based on gene sequences. To include the large number of amino acid sequences of
antibodies whose DNA has not been sequenced and to ensure a maximal fit to rearranged
human Ig variable region genes, we have made a comprehensive comparison of both
protein and nucleotide sequences. The resulting set of 15 primers was able to amplify a
wide range of rearranged antibody variable region genes. Restriction sites included in the …
were based on gene sequences. To include the large number of amino acid sequences of
antibodies whose DNA has not been sequenced and to ensure a maximal fit to rearranged
human Ig variable region genes, we have made a comprehensive comparison of both
protein and nucleotide sequences. The resulting set of 15 primers was able to amplify a
wide range of rearranged antibody variable region genes. Restriction sites included in the …
