A chloride dependent K+ flux induced by N-ethylmaleimide in genetically low K+ sheep and goat erythrocytes
PK Lauf, BE Theg - Biochemical and biophysical research communications, 1980 - Elsevier
PK Lauf, BE Theg
Biochemical and biophysical research communications, 1980•ElsevierIn genetically low K+ but not in high K+ red cells of sheep and goat N-ethylmaleimide
induced a ouabain insensitive K+ flux as measured by tracer influx or net efflux methods.
The augmented K+ flux was observed in Cl− or Br− but not in NO 3−, SO 4 2− or PO 4 2−
media. The action of N-ethylmaleimide was distinct from that of parachloromercuribenzoate
or its sulfonic acid derivative which increased both passive K+ and Na+ movements across
the red cell membrane. The instantaneous selective action of N-ethylmaleimide suggests …
induced a ouabain insensitive K+ flux as measured by tracer influx or net efflux methods.
The augmented K+ flux was observed in Cl− or Br− but not in NO 3−, SO 4 2− or PO 4 2−
media. The action of N-ethylmaleimide was distinct from that of parachloromercuribenzoate
or its sulfonic acid derivative which increased both passive K+ and Na+ movements across
the red cell membrane. The instantaneous selective action of N-ethylmaleimide suggests …
In genetically low K+ but not in high K+ red cells of sheep and goat N-ethylmaleimide induced a ouabain insensitive K+ flux as measured by tracer influx or net efflux methods. The augmented K+ flux was observed in Cl− or Br− but not in NO 3−, SO 4 2− or PO 4 2− media. The action of N-ethylmaleimide was distinct from that of parachloromercuribenzoate or its sulfonic acid derivative which increased both passive K+ and Na+ movements across the red cell membrane. The instantaneous selective action of N-ethylmaleimide suggests that sulfhydryl groups control a K+ Cl− transport system which, associated with the low K+ gene, is apparently functionally silent in adult ruminant red cells.
Elsevier
