[HTML][HTML] Activation of diacylglycerol O-acyltransferase 1 gene results in increased tumor necrosis factor-α gene expression in 3T3-L1 adipocytes
T Hirata, H Unoki, H Bujo, K Ueno, Y Saito - FEBS letters, 2006 - Elsevier
T Hirata, H Unoki, H Bujo, K Ueno, Y Saito
FEBS letters, 2006•ElsevierThe tumor necrosis factor-α (TNF-α) expression has been reported to be largely dependent
on the size of adipocytes. We herein investigated the gene regulation of diacylglycerol O-
acyltransferase (DGAT) in order to clarify the mechanism of TNF-α expression induced in
large adipocytes. 3T3-L1 cells were cultured in the presence of 5mM or 25mM glucose to
generate adipocytes from which the triglyceride content differs. The expression of TNF-α,
DGAT1, and DGAT2 were upregulated in adipocytes cultured with 25mM glucose …
on the size of adipocytes. We herein investigated the gene regulation of diacylglycerol O-
acyltransferase (DGAT) in order to clarify the mechanism of TNF-α expression induced in
large adipocytes. 3T3-L1 cells were cultured in the presence of 5mM or 25mM glucose to
generate adipocytes from which the triglyceride content differs. The expression of TNF-α,
DGAT1, and DGAT2 were upregulated in adipocytes cultured with 25mM glucose …
The tumor necrosis factor-α (TNF-α) expression has been reported to be largely dependent on the size of adipocytes. We herein investigated the gene regulation of diacylglycerol O-acyltransferase (DGAT) in order to clarify the mechanism of TNF-α expression induced in large adipocytes. 3T3-L1 cells were cultured in the presence of 5mM or 25mM glucose to generate adipocytes from which the triglyceride content differs. The expression of TNF-α, DGAT1, and DGAT2 were upregulated in adipocytes cultured with 25mM glucose. Furthermore, knockdown of DGAT1 gene significantly inhibited the TNF-α expression. Finally, the DGAT1 expression levels were closely related to the TNF-α level in 3T3-L1 adipocytes.
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