Human and rabbit paraoxonases: purification, cloning, sequencing, mapping and role of polymorphism in organophosphate detoxification
CE Furlong, LG Costa, C Hassett, RJ Richter… - Chemico-biological …, 1993 - Elsevier
Human and rabbit paraoxonases/arylesterases were purified to homogeneity by
chromatographic and gel electrophoretic/isofocusing procedures coupled with activity stains.
N-terminal and peptide sequence analysis suggested retention of the secretion signal
sequence and allowed design of oligonucleotide probes. The probes were used to isolate a
1294-bp rabbit paraoxonase cDNA clone, which, in turn, was used to isolate three human
cDNA clones. Comparison of rabbit and human protein and cDNA sequences indicated a …
chromatographic and gel electrophoretic/isofocusing procedures coupled with activity stains.
N-terminal and peptide sequence analysis suggested retention of the secretion signal
sequence and allowed design of oligonucleotide probes. The probes were used to isolate a
1294-bp rabbit paraoxonase cDNA clone, which, in turn, was used to isolate three human
cDNA clones. Comparison of rabbit and human protein and cDNA sequences indicated a …
