The influence of protein stability on the adsorption and desorptionbehavior to surfaces with
fundamentally different properties (negativelycharged, positively charged, hydrophilic, and
hydrophobic) was examined bysurface plasmon resonance measurements. Three
engineered variants of humancarbonic anhydrase II were used that have unchanged
surface properties butlarge differences in stability. The orientation and conformational state
ofthe adsorbed protein could be elucidated by taking all of the followingproperties of the …