Decreased endothelial nitric-oxide synthase (eNOS) activity resulting from abnormal interaction between eNOS and its regulatory proteins in hypoxia-induced …
In the pulmonary artery isolated from 1-week hypoxia-induced pulmonary hypertensive rats,
endothelial NO production stimulated by carbachol was decreased significantly in in situ
visualization using diaminofluorescein-2 diacetate and also in cGMP content. This change
was followed by the decrease in carbachol-induced endothelium-dependent relaxation.
Protein expression of endothelial NO synthase (eNOS) and its regulatory proteins, caveolin-
1 and heat shock protein 90, did not change in the hypoxic pulmonary artery, indicating that …
endothelial NO production stimulated by carbachol was decreased significantly in in situ
visualization using diaminofluorescein-2 diacetate and also in cGMP content. This change
was followed by the decrease in carbachol-induced endothelium-dependent relaxation.
Protein expression of endothelial NO synthase (eNOS) and its regulatory proteins, caveolin-
1 and heat shock protein 90, did not change in the hypoxic pulmonary artery, indicating that …
