Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal, gastric and hepatic tissues

C Rubie, K Kempf, J Hans, T Su, B Tilton… - Molecular and cellular …, 2005 - Elsevier
Careful normalization is essential for the accurate quantitation of mRNA levels in biopsy-
sized tissue samples. Commonly, normalization of the target gene with an endogenous
standard, mainly housekeeping genes (HKGs), is applied. However, differences in the
expression levels of endogenous reference genes have been reported between different
tissues and pathological states. Therefore, we were challenged to identify a set of
endogenous reference genes whose mRNA expression levels would not change …