Reversible neural stem cell niche dysfunction in a model of multiple sclerosis
S Rasmussen, J Imitola, A Ayuso‐Sacido… - Annals of …, 2011 - Wiley Online Library
Annals of neurology, 2011•Wiley Online Library
Objective The subventricular zone (SVZ) of the brain constitutes a niche for neural stem and
progenitor cells that can initiate repair after central nervous system (CNS) injury. In a
relapsing‐remitting model of experimental autoimmune encephalomyelitis (EAE), the neural
stem cells (NSCs) become activated and initiate regeneration during acute disease, but lose
this ability during the chronic phases of disease. We hypothesized that chronic microglia
activation contributes to the failure of the NSC repair potential in the SVZ. Methods Using …
progenitor cells that can initiate repair after central nervous system (CNS) injury. In a
relapsing‐remitting model of experimental autoimmune encephalomyelitis (EAE), the neural
stem cells (NSCs) become activated and initiate regeneration during acute disease, but lose
this ability during the chronic phases of disease. We hypothesized that chronic microglia
activation contributes to the failure of the NSC repair potential in the SVZ. Methods Using …
Objective
The subventricular zone (SVZ) of the brain constitutes a niche for neural stem and progenitor cells that can initiate repair after central nervous system (CNS) injury. In a relapsing‐remitting model of experimental autoimmune encephalomyelitis (EAE), the neural stem cells (NSCs) become activated and initiate regeneration during acute disease, but lose this ability during the chronic phases of disease. We hypothesized that chronic microglia activation contributes to the failure of the NSC repair potential in the SVZ.
Methods
Using bromodeoxyuridine injections at different time points during EAE, we quantified the number of proliferating and differentiating progenitors, and evaluated the structure of the SVZ by electron microscopy. In vivo minocycline treatment during EAE was used to address the effect of microglia inactivation on SVZ dysfunction.
Results
In vivo treatment with minocycline, an inhibitor of microglia activation, increases stem cell proliferation in both naive and EAE animals. Minocycline treatment decreases cortical and periventricular pathology in the chronic phase of EAE, improving the proliferation of Sox2 stem cells and NG2 oligodendrocyte precursors cells originating in the SVZ and their differentiation into mature oligodendrocytes.
Interpretation
These data suggest that failure of repair observed during chronic EAE correlates with microglia activation and that treatments targeting chronic microglial activation have the potential for enhancing repair in the CNS. ANN NEUROL 2011
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