[HTML][HTML] Conserved balance of hepatocyte nuclear DNA content in mononuclear and binuclear hepatocyte populations during the course of chronic viral hepatitis
H Toyoda, T Kumada, O Bregerie… - World Journal of …, 2006 - ncbi.nlm.nih.gov
H Toyoda, T Kumada, O Bregerie, C Brechot, C Desdouets
World Journal of Gastroenterology: WJG, 2006•ncbi.nlm.nih.govAIM: To analyze the percentages of hepatocytes with increased nuclear DNA content, ie,
tetraploid (4n) and octoploid (8n) nuclei, and then compared mononuclear and binuclear
hepatocyte populations. METHODS: The percentages of mononuclear diploid (2n), 4n, and
8n hepatocytes and those of binuclear 2× 2n, 2× 4n, and 2× 8n hepatocytes were
determined with a method that can simultaneously measure hepatocyte nuclear DNA
content and binuclearity in 62 patients with chronic hepatitis B or C. The percentage of 4n …
tetraploid (4n) and octoploid (8n) nuclei, and then compared mononuclear and binuclear
hepatocyte populations. METHODS: The percentages of mononuclear diploid (2n), 4n, and
8n hepatocytes and those of binuclear 2× 2n, 2× 4n, and 2× 8n hepatocytes were
determined with a method that can simultaneously measure hepatocyte nuclear DNA
content and binuclearity in 62 patients with chronic hepatitis B or C. The percentage of 4n …
Abstract
AIM: To analyze the percentages of hepatocytes with increased nuclear DNA content, ie, tetraploid (4n) and octoploid (8n) nuclei, and then compared mononuclear and binuclear hepatocyte populations.
METHODS: The percentages of mononuclear diploid (2n), 4n, and 8n hepatocytes and those of binuclear 2× 2n, 2× 4n, and 2× 8n hepatocytes were determined with a method that can simultaneously measure hepatocyte nuclear DNA content and binuclearity in 62 patients with chronic hepatitis B or C. The percentage of 4n and 8n hepatocytes in the mononuclear hepatocyte population was compared with the percentage of 2× 4n and 2× 8n hepatocytes in the binuclear hepatocyte population.
RESULTS: The percentages of 4n and 8n hepatocytes in mononuclear hepatocytes and 2× 4n and 2× 8n hepatocytes in binuclear hepatocytes were similar, regardless of the activity or fibrosis grade of chronic hepatitis and regardless of the infecting virus.
CONCLUSION: The distribution of nuclear DNA content within mononuclear and binuclear hepatocyte populations was conserved during the course of chronic viral hepatitis.
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