[HTML][HTML] Analysis of polymorphic residues reveals distinct enzymatic and cytotoxic activities of the Streptococcus pyogenes NAD+ glycohydrolase
S Chandrasekaran, J Ghosh, GC Port, E Koh… - Journal of Biological …, 2013 - ASBMB
The Streptococcus pyogenes NAD+ glycohydrolase (SPN) is secreted from the bacterial cell
and translocated into the host cell cytosol where it contributes to cell death. Recent studies
suggest that SPN is evolving and has diverged into NAD+ glycohydrolase-inactive variants
that correlate with tissue tropism. However, the role of SPN in both cytotoxicity and niche
selection are unknown. To gain insight into the forces driving the adaptation of SPN, a
detailed comparison of representative glycohydrolase activity-proficient and-deficient …
and translocated into the host cell cytosol where it contributes to cell death. Recent studies
suggest that SPN is evolving and has diverged into NAD+ glycohydrolase-inactive variants
that correlate with tissue tropism. However, the role of SPN in both cytotoxicity and niche
selection are unknown. To gain insight into the forces driving the adaptation of SPN, a
detailed comparison of representative glycohydrolase activity-proficient and-deficient …