Tacrolimus (FK506) limits accumulation of granulocytes and platelets and protects against brain damage after transient focal cerebral ischemia in rat
T Noto, Y Furuichi, M Ishiye, N Matsuoka… - Biological and …, 2007 - jstage.jst.go.jp
T Noto, Y Furuichi, M Ishiye, N Matsuoka, I Aramori, S Mutoh, T Yanagihara
Biological and Pharmaceutical Bulletin, 2007•jstage.jst.go.jpMATERIALS AND METHODS Surgical Procedure Experiments were carried out using male
Wistar rats (8—10 week-old, 300—395g) purchased from Charles River Japan (Hino,
Japan). This study was approved by the Experimental Laboratory Animal Committee of
Fujisawa Pharmaceutical Co., Ltd.(now Astellas Pharmaceutical Inc.). The procedure for
transient MCA occlusion was as described by Koizumi et al. 19) Briefly, each rat was
anesthetized with inhalation of 4.0% halothane in oxygen–nitrogen (30% oxygen and 70 …
Wistar rats (8—10 week-old, 300—395g) purchased from Charles River Japan (Hino,
Japan). This study was approved by the Experimental Laboratory Animal Committee of
Fujisawa Pharmaceutical Co., Ltd.(now Astellas Pharmaceutical Inc.). The procedure for
transient MCA occlusion was as described by Koizumi et al. 19) Briefly, each rat was
anesthetized with inhalation of 4.0% halothane in oxygen–nitrogen (30% oxygen and 70 …
MATERIALS AND METHODS
Surgical Procedure Experiments were carried out using male Wistar rats (8—10 week-old, 300—395g) purchased from Charles River Japan (Hino, Japan). This study was approved by the Experimental Laboratory Animal Committee of Fujisawa Pharmaceutical Co., Ltd.(now Astellas Pharmaceutical Inc.).
The procedure for transient MCA occlusion was as described by Koizumi et al. 19) Briefly, each rat was anesthetized with inhalation of 4.0% halothane in oxygen–nitrogen (30% oxygen and 70% nitrogen). A mixture of 1.5% halothane and oxygen–nitrogen was used to maintain anesthesia during operation. Each animal was placed in the supine position and a midline incision was made in the skin of the neck. The right common carotid artery (CCA) was carefully exposed without
jstage.jst.go.jp
