[HTML][HTML] Pancreatic β-Cell-specific Targeted Disruption of Glucokinase Gene: DIABETES MELLITUS DUE TO DEFECTIVE INSULIN SECRETION TO GLUCOSE (∗)

Y Terauchi, H Sakura, K Yasuda, K Iwamoto… - Journal of Biological …, 1995 - ASBMB
Mice carrying a null mutation in the glucokinase (GK) gene in pancreatic β-cells, but not in
the liver, were generated by disrupting the β-cell-specific exon. Heterozygous mutant mice
showed early-onset mild diabetes due to impaired insulin-secretory response to glucose.
Homozygotes showed severe diabetes shortly after birth and died within a week. GK-
deficient islets isolated from homozygotes showed defective insulin secretion in response to
glucose, while they responded to other secretagogues: almost normally to arginine and to …