[HTML][HTML] Activation of hypoxia-inducible factor-1; definition of regulatory domains within the α subunit
CW Pugh, JF O'Rourke, M Nagao, JM Gleadle… - Journal of Biological …, 1997 - ASBMB
Hypoxia-inducible factor-1 (HIF-1), a heterodimeric DNA binding complex composed of two
basic-helix-loop-helix Per-AHR-ARNT-Sim proteins (HIF-1α and− 1β), is a key component of
a widely operative transcriptional response activated by hypoxia, cobaltous ions, and iron
chelation. To identify regions of HIF-1 subunits responsible for oxygen-regulated activity, we
constructed chimeric genes in which portions of coding sequence from HIF-1 genes were
either linked to a heterologous DNA binding domain or encoded between such a DNA …
basic-helix-loop-helix Per-AHR-ARNT-Sim proteins (HIF-1α and− 1β), is a key component of
a widely operative transcriptional response activated by hypoxia, cobaltous ions, and iron
chelation. To identify regions of HIF-1 subunits responsible for oxygen-regulated activity, we
constructed chimeric genes in which portions of coding sequence from HIF-1 genes were
either linked to a heterologous DNA binding domain or encoded between such a DNA …
