Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular …

J Lötvall, AF Hill, F Hochberg, EI Buzás… - Journal of …, 2014 - Wiley Online Library
J Lötvall, AF Hill, F Hochberg, EI Buzás, D Di Vizio, C Gardiner, YS Gho, IV Kurochkin
Journal of extracellular vesicles, 2014Wiley Online Library
Secreted membrane‐enclosed vesicles, collectively called extracellular vesicles (EVs),
which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and
other EV subsets, encompass a very rapidly growing scientific field in biology and medicine.
Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from
non‐vesicular components for functional studies, and therefore there is a need to establish
guidelines for analyses of these vesicles and reporting of scientific studies on EV biology …
Secreted membrane‐enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non‐vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.
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