[HTML][HTML] Visualization of altered replication dynamics after DNA damage in human cells

CJ Merrick, D Jackson, JFX Diffley - Journal of Biological Chemistry, 2004 - ASBMB
Eukaryotic cells respond to DNA damage within the S phase by activating an intra-S
checkpoint: a response that includes reducing the rate of DNA synthesis. In yeast cells this
can occur via checkpoint-dependent inhibition of origin firing and stabilization of ongoing
forks, together with a checkpoint-independent slowing of fork movement. In higher
eukaryotes, however, the mechanism by which DNA synthesis is reduced is less clear. We
have developed strategies based on DNA fiber labeling that allow the quantitative …