Determination of arginase activity in macrophages: a micromethod

IM Corraliza, ML Campo, G Soler, M Modolell - Journal of immunological …, 1994 - Elsevier
We propose a modification of Schimke's method for urea determination as a valuable
micromethod for measuring arginase in activated macrophages. The method exhibits the
following advantages:(a) it uses small amounts of samples (approximately 25 000
macrophages per assay);(b) it does not interfere with other related metabolites that are also
present in the activated macrophage such as citrulline or arginine;(c) saturating
concentrations of the substrate arginine can be used; and (d) it is much more sensitive than …