Objective Sample manipulation to obtain isolated granulocytes represents a key, and often
necessary, step in the in vitro studies. We investigated by the means of flow cytometry and
microscopic techniques (both optical microscopy [OM] and scanning electron microscopy
[SEM]), the granulocyte-endothelium adhesion and the role of sample manipulation.
Methods By means of a co-culture method, we have analysed the adhesion of human
leukocytes, originated from two different blood samples (fresh venous blood [FB] and buffy …