A sensitive HPLC–MS/MS method for the determination of dolutegravir in human plasma
C Bennetto-Hood, G Tabolt, P Savina… - … of Chromatography B, 2014 - Elsevier
A sensitive liquid chromatography tandem mass spectrometry (LC–MS/MS) assay was
developed and validated to facilitate the assessment of clinical pharmacokinetics of
dolutegravir (DTG) in plasma samples. This work describes an assay system requiring only
a 20 μL aliquot of human plasma that is subjected to a simple acetonitrile protein
precipitation containing a stably labeled isotope of DTG used as an internal standard.
Chromatography was performed on an XBridge C18, 2.1 mm× 50 mm, reversed phase …
developed and validated to facilitate the assessment of clinical pharmacokinetics of
dolutegravir (DTG) in plasma samples. This work describes an assay system requiring only
a 20 μL aliquot of human plasma that is subjected to a simple acetonitrile protein
precipitation containing a stably labeled isotope of DTG used as an internal standard.
Chromatography was performed on an XBridge C18, 2.1 mm× 50 mm, reversed phase …