Infection with a virus generates a polyclonal immune response with broad alloreactive potential
H van den Heuvel, EMW van der Meer-Prins… - Human immunology, 2019 - Elsevier
Virus-specific T cells have been shown to cross-react with allogeneic HLA (allo-HLA) at a
clonal level. However, the impact of a single virus on the allorepertoire has never been
investigated at the polyclonal level. We made an inventory of the incidence and specificity of
allo-HLA-cross-reactive-virus-specific CD8+ T cells in 24 healthy individuals. T cells were
stained for 25 virus-specific tetramers, and mixed-lymphocyte reactions were performed
against a panel of HLA-typed allostimulators. Allospecificity was confirmed by IFNγ-ELISA …
clonal level. However, the impact of a single virus on the allorepertoire has never been
investigated at the polyclonal level. We made an inventory of the incidence and specificity of
allo-HLA-cross-reactive-virus-specific CD8+ T cells in 24 healthy individuals. T cells were
stained for 25 virus-specific tetramers, and mixed-lymphocyte reactions were performed
against a panel of HLA-typed allostimulators. Allospecificity was confirmed by IFNγ-ELISA …
