Simultaneously quantitative measurement of comprehensive profiles of esterified and non-esterified fatty acid in plasma of type 2 diabetic patients
L Yi, J He, Y Liang, D Yuan, H Gao, H Zhou - Chemistry and Physics of …, 2007 - Elsevier
Fatty acids, having intimate relationship with type 2 diabetes mellitus (DM2), are not only the
main energy source as nutrients, but also signaling molecules in insulin secretion. In this
work, we developed a two-step rapid method to comprehensive profiling of esterified fatty
acid (EFA) and non-esterified fatty acid (NEFA) using KOH–CH3OH to methylate EFA
followed by H2SO4–CH3OH to methylate NEFA. Its applications to fatty acids profiling of
type 2 diabetic patients and health controls were also presented. The t-test results informed …
main energy source as nutrients, but also signaling molecules in insulin secretion. In this
work, we developed a two-step rapid method to comprehensive profiling of esterified fatty
acid (EFA) and non-esterified fatty acid (NEFA) using KOH–CH3OH to methylate EFA
followed by H2SO4–CH3OH to methylate NEFA. Its applications to fatty acids profiling of
type 2 diabetic patients and health controls were also presented. The t-test results informed …