[HTML][HTML] N-terminal acetylation and the N-end rule pathway control degradation of the lipid droplet protein PLIN2
Perilipin 2 (PLIN2) is a major lipid droplet (LD)-associated protein that regulates intracellular
lipid homeostasis and LD formation. Under lipid-deprived conditions, the LD-unbound (free)
form of PLIN2 is eliminated in the cytosol by an as yet unknown ubiquitin (Ub)-proteasome
pathway that is associated with the N-terminal or near N-terminal residues of the protein.
Here, using HeLa, HEK293T, and HepG2 human cell lines, cycloheximide chase, in vivo
ubiquitylation, split-Ub yeast two-hybrid, and chemical cross-linking–based reciprocal co …
lipid homeostasis and LD formation. Under lipid-deprived conditions, the LD-unbound (free)
form of PLIN2 is eliminated in the cytosol by an as yet unknown ubiquitin (Ub)-proteasome
pathway that is associated with the N-terminal or near N-terminal residues of the protein.
Here, using HeLa, HEK293T, and HepG2 human cell lines, cycloheximide chase, in vivo
ubiquitylation, split-Ub yeast two-hybrid, and chemical cross-linking–based reciprocal co …