Concomitant translocation of Purα with its binding proteins (PurBPs) from nuclei to cytoplasm during neuronal development
LH Zeng, K Okamura, H Tanaka, N Miki, CH Kuo - Neuroscience research, 2005 - Elsevier
Two Purα-binding proteins (PurBPs) were found in nuclear extract from mouse brain during
P4–P10 by the overlay assay. At P14, they were decreased significantly in nuclear extract
and increased in the S3 fraction, indicating their dynamic translocation during development.
Western blot analysis also demonstrated concomitant translocation of Purα with the PurBPs
during P7–P14, when neuronal circuit proceeds. Immunocytochemical study with cultured
hippocampal neurons from rat E18 confirmed that nuclear Purα was translocated to …
P4–P10 by the overlay assay. At P14, they were decreased significantly in nuclear extract
and increased in the S3 fraction, indicating their dynamic translocation during development.
Western blot analysis also demonstrated concomitant translocation of Purα with the PurBPs
during P7–P14, when neuronal circuit proceeds. Immunocytochemical study with cultured
hippocampal neurons from rat E18 confirmed that nuclear Purα was translocated to …