A, Male and female mice (8 weeks old) were subjected to HFpEF treatment (high-fat diet+ N [w]-nitro-L-arginine methyl ester [HFD+ L-NAME]) vs normal chow for 5 weeks. Top, Body weight (BW; n= 13, 20, 13, 20 mice from left to right groups), systolic blood pressure (SBP) and diastolic blood pressure (DBP; n= 5, 5, 5, 7 mice; each value is the mean of 3 independent measurements), and blood glucose level after intraperitoneal injection of glucose (2 mg/g BW; n= 5 mice per group). Middle, Left ventricular ejection fraction (EF; n= 5, 8, 5, 8 mice), ratio between mitral E wave and e’wave (E/e’; n= 10 mice per group), and ratio of heart weight to tibia length (HW/TL; n= 5, 7, 5, 7 mice). Bottom, Left ventricular remodeling index (LVRI; ratio of left ventricular mass to left ventricular end-diastolic internal diameter [LVM/LVIDd]; n= 5, 8, 5, 8 mice), running distance during exercise exhaustion test (n= 8 mice per group), and lung water assessed by subtracting dry lung weight from wet lung (Continued)